STRUCTURE OF THE ACID-LABILE GALACTOSYL PHOSPHATE-CONTAINING O-ANTIGEN OF THE BACTERIUM PROTEUS VULGARIS OX19 (SEROGROUP O1) USED IN WEIL-FELIX TEST
aN. D. Zelinsky Institute of Organic
Chemistry, Russian Academy of Sciences, Moscow, Russia
bCenter of Microbiology and Virology, Polish Academy of Sciences,
Lodz, Poland
cCentral Research Laboratory, Akita University,
School of Medicine, Japan
KEYWORDS: lipopolysaccharide, O-antigen, bacterial polysaccharide, structure, serological cross-reactivity, rickettsiosis, Weil-Felix test, Proteus vulgaris, Rickettsia
Biochemistry/Moscow, 1997, v. 62(5), pp. 461-468
See
also more general review in FEBS Lett., 1997, v. 411,
pp. 221-224
Structure of the O-specific polysaccharide chain of Proteus vulgaris OX19 lipopolysaccharide which determines the O1 specificity of Proteus and used in Weil-Felix test for diagnostics of rickettssiosis, was established. On the basis of 1H- and 13C-NMR spectroscopy, including two-dimensional correlation spectroscopy (COSY), H-detected 1H,13C heteronuclear multiple-quantum coherence (HMQC), and rotating-frame nuclear Overhauser effect spectroscopy (ROESY), it was found that the polysaccharide consists of repeating branched pentasaccharide repeating units containing D-galactose, 2-acetamido-2-deoxy-D-glucose, 2-acetamido-2-deoxy-D-galactose, and 2-acetamido-2,6-dideoxy-D-glucose (QuiNAc, two residues), which are connected to each other via a phosphate group (P):
aQuipNAc
|3
-4)aGalpNAc(1-4)aGalp(1-P-4)aQuipNAc(1-3)bGlcpNAc(1- |
The polysaccharide is acid-labile, the glycosyl phosphate linkage being cleaved at pH 4.5 (100o) to give a phosphorylated pentasaccharide with a galactose residue at the reducing end. Structural analysis of the oligosaccharide and a product of its dephosphorylation with 48% hydrofluoric acid using 1H- and 13C-NMR spectroscopy and electrospray ionization mass spectrometry confirmed the structure of the polysaccharide.
 Наука : Proteus |
 Публикации : Proteus |
 Дом : Наука |