STRUCTURES OF NEW ACIDIC O-SPECIFIC POLYSACCHARIDES OF THE BACTERIUM PROTEUS MIRABILIS SEROGROUPS O26 AND O30

A.S. Shashkov^a, F.V. Toukach^a, N.A. Paramonov^a, A. Ziolkowski^b, S.N. Senchenkova^a, W. Kaca^b, Y.A. Knirel^a*

^aN. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
^bCenter of Microbiology and Virology, Polish Academy of Sciences, Lodz, Poland

KEYWORDS: Lipopolysaccharide; O-Antigen structure; Bacterial acidic polysaccharide; Hexuronic acid; Lysine, L-; (Proteus mirabilis)

FEBS Lett., 1996, v. 386, pp. 247-251

DOI: 10.1016/0014-5793(96)00454-1

The polysaccharide chains of the Proteus mirabilis serogroups O26 and O30 lipopolysaccharides were studied using sugar and methylation analysis and ¹H and ¹³C NMR spectroscopy, including two-dimensional correlation spectroscopy and rotating-frame NOE spectroscopy. The polysaccharides were found to be acidic due to the presence of D-galacturonic acid and its amide with L-lysine in serogroup O26 or D-glucuronic acid in serogroup O30, and the structures of their tetrasaccharide repeating units were established. The O26-specific polysaccharide is structurally and serologically related to the O-specific polysaccharide of P. mirabilis O28, which includes amides of DGalA with L-lysine and L-serine [Radziejewska-Lebrecht, J. et al. (1995) Eur. J. Biochem. 230, 705-712].

Наука : Proteus

Публикации : Proteus

Дом : Наука