STRUCTURE OF THE O-SPECIFIC POLYSACCHARIDE OF THE BACTERIUM PROTEUS MIRABILIS O13 CONTAINING A NOVEL AMIDE OF D-GALACTURONIC ACID WITH N^e-(1-CARBOXYETHYL)LYSINE

A.S. Shashkov^a, F.V. Toukach^a, S.N. Senchenkova^a, A. Ziolkowski^b, N.A. Paramonov^a, W. Kaca^b, Y.A. Knirel^a*, N.K. Kochetkov^a

^aN. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
^bCenter of Microbiology and Virology, Polish Academy of Sciences, Lodz, Poland

KEYWORDS: O-antigen, bacetrial polysaccharide, D-galacturonic acid, N^e-(1-carboxyethyl)lysine, structure, NMR spectroscopy, Proteus mirabilis.

Biochemistry/Moscow, 1997, v. 62(5), pp. 509-513,

An acidic O-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of the bacterium Proteus mirabilis O13 and found to contain D-galactose, 2-acetamido-2-deoxy-D-glucose, and N^e-(1-carboxyethyl)-N*-(D-galacturonoyl)lysine. On the basis of full acid hydrolysis and ¹H- and ¹³C-NMR spectroscopy, including two-dimensional correlation spectroscopy (COSY), H-detected heteronuclear ¹H,¹³C multi-quantum coherence (HMQC), and rotating-frame nuclear Overhauser effect spectroscopy (ROESY), the following structure of the branched trisaccharide repeating unit of the polysaccharide was established:

Ne-(1-carboxyEt)Lys(N-6)aDGalpA
                          |4
       -3)bDGlcpNAc(1-3)aDGalp(1-

Наука : Proteus

Публикации : Proteus

Дом : Наука