STRUCTURAL AND SEROLOGICAL STUDIES OF THE O-SPECIFIC POLYSACCHARIDE OF THE BACTERIUM PROTEUS MIRABILIS O10 CONTAINING L-ALTRURONIC ACID, A NEW COMPONENT OF O-ANTIGENS

A.S. Swierzko^a, A.S. Shashkov^b, S.N. Senchenkova^b, F.V. Toukach^b, A. Ziolkowski^a, M. Cedzynski^a, N.A. Paramonov^b, W. Kaca^a, Y.A. Knirel^b*

^aCenter of Microbiology and Virology, Polish Academy of Sciences, Lodz, Poland
^bN. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia

KEYWORDS: lipopolysaccharide, O-antigen, polysaccharide, uronic acids, structure, NMR spectroscopy, Proteus mirabilis

FEBS Lett., 1996, v. 398, pp. 297-302
Also in Biochemistry/Moscow, 1996, v. 61(9), pp. 1100-1105

DOI: 10.1016/S0014-5793(96)01061-7

An acidic O-specific polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of the bacterium Proteus mirabilis O10 and studied by full acid hydrolysis before and after carboxyl reduction and by ¹H- and ¹³C-NMR spectroscopy, including two-dimensional correlation spectroscopy (COSY), H-detected heteronuclear ¹H,¹³C multi-quantum coherence (HMQC), and rotating-frame nuclear Overhauser effect spectroscopy (ROESY). It was found that the O-specific polysaccharide contains one residue each of 2-acetamido-2-deoxy-D-glucose, 2-acetamido-2-deoxy-D-galactose, D-galacturonic acid, and L-altruronic acid, and the following structure of the branched tetrasaccharide repeating unit of the polysaccharide was esteblished:

   aLAltpA
      |3
-4)aDGalpNAc(1-3)aDGalpA(1-3)aDGlcpNAc(1-

Наука : Proteus

Публикации : Proteus

Дом : Наука