STRUCTURE AND GENE CLUSTER OF THE O-POLYSACCHARIDE OF YERSINIA ROHDEI H274-36/78

O.V. Sizova^a, A.S. Shashkov^a, A.S. Dmitrenok^a, Ph.V. Toukach^a*, Y.A. Knirel^a, R.Z. Shaikhutdinova^b, S.A. Ivanov^a, A.A. Kislichkina^b, A.G. Bogun^b, S.V. Dentovskaya^b

^aN. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
^bState Research Center for Applied Microbiology and Biotechnology, Obolensk, Russia

KEYWORDS: Yersinia rohdei; O-specific polysaccharide; O-antigen gene cluster; lipopolysaccharide; yersiniose; bacterial polysaccharide structure; NMR simulation

International Journal of Biological Macromolecules, 2019, т. 122, стр. 555-561

DOI: 10.1016/j.ijbiomac.2018.10.189, PMID: 30385338

A branched O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Yersinia rohdei H274-36/78 and found to contain D-rhamnose, D-mannose, and 3,6-dideoxy-4-C-[(S)-1-hydroxyethyl]-D-xylo-hexose called yersiniose A (Yer). Partial acid hydrolysis of the O-polysaccharide eliminated Yer residues to give a modified linear polysaccharide. Studies by sugar analysis and 1H and 13C NMR spectroscopy, including computational NMR analysis, enabled structure elucidation of a hexasaccharide repeating unit of the O-polysaccharide having two Yer residues attached as monosaccharide side chains. The O-antigen gene cluster of Y. rohdei H274-36/78 located between JUMPStart and galF genes contained putative genes for synthesis of precursors of two O-antigen constituents, GDP-D-Man and GDP-D-Rha, whereas genes responsible for synthesis of CDP-Yer were within the chromosome outside the O-antigen gene cluster. Glycosyltransferase genes and ABC 2 transporter genes were present in the O-antigen gene cluster, and hence the structure established is consistent with the polysaccharide synthesis gene content of the genome.

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Дом : Наука