STRUCTURE ELUCIDATION AND GENE CLUSTER ANNOTATION OF THE O-ANTIGEN OF STUTZERIMONAS CHLORITIDISMUTANS NP21570 WITH 2-ACETAMIDO-2-DEOXY-D-MANNURONIC ACID

A.V. Perepelov^a, A.A. Kasimova^a, A.V. Filatov^a, Ph.V. Toukach^a, T.L. Babich^b, N.M. Popova^c, D.V. Krivonos^d,e, A.V. Safonov^f

^aN.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
^bResearch Center of Biotechnology, Russian Academy of Sciences, Moscow, Russia
^cA.N. Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, Moscow, Russia
^dResearch Institute for Systems Biology and Medicine, Moscow, Russia
^eMoscow Center for Advanced Studies, Moscow, Russia
^fV. Vernadsky Institute of Geochemistry and Analytical Chemistry, Russian Academy of Sciences, Moscow, Russia

KEYWORDS: Azospirillum brasilense; Bacterial polysaccharide structure; Lipopolysaccharide; O-polysaccharide; NMR simulation

Carbohydrate Research, 2026, т. 567, ID. 109993

DOI: 10.1016/j.carres.2026.109993

The O-polysaccharide (O-antigen) of Stutzerimonas chloritidismutans NP21570 isolated from deep saline aquifer (1570 m) near Dimitrovgrad, Russian Federation, was studied by chemical methods, including component analyses, along with ¹H and ¹³C NMR spectroscopy and NMR-based structure matching. The following structure of linear disaccharide repeating O-unit, containing two O-acetyl groups, one of them is about 40%, another one is 60%, was established:

-4)-a-D-ManpNAcA-(1-4)-b-D-MAnpNAc3Ac6Ac-(1-

.

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Публикации : прочее

Дом : Наука