STRUCTURE OF THE O-POLYSACCHARIDE AND SEROLOGICAL CROSS-REACTIVITY OF THE LIPOPOLYSACCHARIDE OF PROVIDENCIA ALCALIFACIENS O32 CONTAINING N-ACETYLISOMURAMIC ACID

Ivan S. Bushmarinov^a, Olga G. Ovchinnikova^a*, Nina A. Kocharova^a, Filip V. Toukach^a, Agnieszka Torzewska^b, Alexander S. Shashkov^a, Yuriy A. Knirel^a and Antoni Rozalski^b

^aN. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
^bDepartment of Immunobiology of Bacteria, Institute of Microbiology and Immunology, University of Lodz, Poland

KEYWORDS: Providencia alcalifaciens, O-antigen, lipopolysaccharide, polysaccharide structure, isomuramic acid, C-13-NMR data, Proteus, classification

Carbohydrate Research, 2007, т. 342(2), стр. 268-273

DOI: 10.1016/j.carres.2006.11.027

The O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide of Providencia alcalifaciens O32 and studied by sugar and methylation analyses, solvolysis with triflic acid, ¹H and ¹³C NMR spectroscopy, including two-dimensional ¹H,¹H COSY, TOCSY, ROESY, H-detected ¹H,¹³C HSQC and HMBC experiments. It was found that the polysaccharide has a branched tetrasaccharide repeating unit containing 2-acetamido-3-O+[(S)-1-carboxyethyl]-2-deoxy-D-glucose (D-Glc-NAc3Slac, N-acetylisomuramic acid) with the following structure:

                   bDGlcp         S-Lac
                      |4             |3
->3)aLFucpNAc(1->3)aDGlcpNAc(1->6)aDGlcpNAc(1->

Serological studies with O-antisera showed antigenic relationships between P. alcalifaciens O32 and O29 as well as several other Providencia and Proteus strains sharing putative epitopes on the O-polysaccharides.

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