NEW STRUCTURE FOR THE O-POLYSACCHARIDE OF PROVIDENCIA ALCALIFACIENS O27 AND REVISED STRUCTURE FOR THE O-POLYSACCHARIDE OF PROVIDENCIA STUARTII O43

Olga G. Ovchinnikova ^a, Ivan S. Bushmarinov ^a, Nina A. Kocharova ^a, Filipp V. Toukach ^a, M. Wykrota^b, Alexander S. Shashkov ^a, Yuriy A. Knirel ^a and Antoni Rozalski ^b

^aN. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
^bDepartment of Immunobiology of Bacteria, Institute of Microbiology and Immunology, University of Lodz, Poland

KEYWORDS: lipopolysaccharide, O-polysaccharide, bacterial polysaccharide structure, Providencia stuartii, Providencia alcalifaciens

Carbohydrate Research, 2007, т. 342(8), стр. 1116-1121

DOI: 10.1016/j.carres.2007.02.028

The O-polysaccharide was obtained by mild acid degradation of the lipopolysaccharide from Providencia alcalifaciens O27 and studied by sugar and methylation analyses along with ¹H and ¹³C NMR spectroscopy, including 2D ¹H,¹H COSY, TOCSY, ROESY, H-detected ¹H,¹³C HSQC, and HMBC experiments. It was found that the polysaccharide is built up of linear partially O-acetylated tetrasaccharide repeating units and has the following structure:

  70% AcO
        |6
-4)-b-DGlcp(1-3)-b-D-GalpNAc(1-2)-a-D-Rhap4NFo(1-4)-a-?-GlcpA(1-

where Qui4NFo stands for 4-formamido-4,6-dideoxyglucose (4-formamido-4-deoxyquinovose). The O-polysaccharide structure of Providencia stuartii O43 established earlier was revised with respect to the configuration of the constituent 4-amino-4,6-dideoxyhexose (from Rha4N to Qui4N).

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