Abstract: Structural and serological studies O-polysaccharides of Edwardsiella tarda

STRUCTURES OF A UNIQUE O-POLYSACCHARIDE OF EDWARDSIELLA TARDA PCM 1153 CONTAINING AN AMIDE OF GALACTURONIC ACID WITH 2-AMINOPROPANE-1,3-DIOL AND AN ABEQUOSE-CONTAINING O-POLYSACCHARIDE SHARED BY E. TARDA PCM 1145, PCM 1151 AND PCM 1158

E. Katzenellenbogen^a, N.A. Kocharova^b, Ph.V. Toukach^b, S. Górska^a, M. Bogulska^a, A. Gamian^a,c, Y.A. Knirel^b

^aL. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Weigla, Wroclaw, Poland
^bN. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia

^cDepartment of Medical Biochemistry, Wroclaw Medical University, Wroclaw, Poland

KEYWORDS: Edwardsiella tarda; Lipopolysaccharide; O-antigen; Bacterial polysaccharide structure; Abequose; 2-Amino-2-deoxyglycerol

Carbohydrate Research, 2012, v. 355, pp. 56-62

DOI: 10.1016/j.carres.2012.04.004

Lipopolysaccharides of four strains of Edwardsiella tarda were degraded by mild acid hydrolysis, and the released O-polysaccharides were isolated by GPC and studied by sugar and methylation analyses along with ¹H and ¹³C NMR spectroscopy, including 2D ¹H,¹H COSY, TOCSY, ROESY, ¹H,¹³C HMBC, HSQC and HSQC-TOCSY experiments. The O-polysaccharide from E. tarda PCM 1153 was found to contain D-GalA, D-GlcNAc, D-Gal and 2-amino-1,3-propanediol (GroN). In the tetrasaccharide repeating unit, GroN is amide-linked to one of the GalA residues, and Gal is non-stoichiometrically 2- or 3-O-acetylated (~45% at each position):

    GroN(2                     90%OAc
         |                        |
         6                       2/3
->4)-a-D-GalpA-(1->4)-a-D-Galp-(1->3)-a-D-GalpA-(1->3)-b-D-GlcpNAc-(1->

Three other E. tarda strains examined (PCM 1145, PCM 1151 and PCM 1158) share the following O-polysaccharide structure:

      a-Abep-2-OAc(
          1
          |
          3
->2)-a-D-Manp-(1->4)-a-L-Rhap-(1->3)-a-D-Galp-(1->

where Abe indicates 3,6-dideoxy-D-xylo-hexose (abequose). This structure resembles those of Citrobacter freundii O22 (PCM 1555) and Salmonella enterica O4. In accordance with the structural data, SDS-PAGE and immunoblotting of the lipopolysaccharides with anti-C.freundii O22 serum demonstrated that the O-antigens of the three E. tarda strains are serologically identical to each other and to the O-antigens of C. freundii O22 and S. enterica O4.

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