STRUCTURAL STUDIES OF ARABINAN-RICH PECTIC POLYSACCHARIDES FROM ABIES SIBIRICA L. BIOLOGICAL ACTIVITY OF PECTINS OF A. SIBIRICA

E.G. Shakhmatov^a, Ph.V. Toukach^b, E.A. Michailowa^c, E.N. Makarova^a

^aInstitute of Chemistry, Komi Science Centre, The Urals Branch of the Russian Academy of Sciences, Syktyvkar, Russia
^bN. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
^c Institute of Physiology, Komi Science Centre, The Urals Branch of the Russian Academy of Sciences, Syktyvkar, Russia

KEYWORDS: Abies sibirica; Pectin; Arabinan; Wood greenery; NMR spectroscopy; β-l-Araf

Carbohydrate Polymers, 2014, т. 113, стр. 515-524

DOI: 10.1016/j.carbpol.2014.07.037

Highly branched arabinan-rich pectic polysaccharides, containing 84% of arabinose, was extracted from wood greenery of Abies sibirica L. The structure of arabinan was studied by the 1D and 2D NMR spectroscopy. The macromolecule backbone was represented mainly by RG-I (molar ratio GalA:Rha ~ 1.3:1) patterns with high degree of rhamnose branching. Side chains were comprised of 1,5-linked α-L-Araf residues (the major part of polymer mass), 1,3,5-di-O- and 1,2,3,5-tri-O-linked α-L-Araf residues, confirming the presence of highly branched 1,5-α-L-arabinan. Although most L-Araf were in α-anomeric form, minor terminal β-L-Araf-(1-... was detected. 1,4-β-D-linked Galp residues found in the side chains account for minor AG-I or 1,4-galactan, as compared to arabinan. A tentative structure was proposed.

Polysaccharides obtained from Siberian fir greenery were screened for biological activity. Galacturonan had a strongest stimulating effect on germination and growth rate of seeds, germs and roots of Triticum aestivum, Avena sativa, and Secale cereale.

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