STRUCTURE AND GENE CLUSTER OF THE CAPSULAR POLYSACCHARIDE OF ACINETOBACTER BAUMANNII K104

A.M. Shpirt^a, Z.V. Serpokrylov^a, A.V. Perepelov^a*, A.S. Dmitrenok^a, M.M. Shneider^b, A.V. Popova^c, P.V. Toukach^a, Y.A. Knirel^a

^aN.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia
^bM.M. Shemyakin & Y.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia
^cState Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Russia

KEYWORDS: Acinetobacter baumannii; Сapsular polysaccharide; K locus; Structure; Gene cluster

Carbohydrate Research, 2025

DOI: 10.1016/j.carres.2025.109745

Structure of the Acinetobacter baumannii MAR20-4513 (K104) capsular polysaccharide (CPS) was established using Smith degradation, ¹H and ¹³C NMR spectroscopy, including two-dimensional homonuclear ¹H,¹H COSY, TOCSY, ROESY and heteronuclear ¹H,¹³C HSQC and HMBC experiments, and modeling. The K104 CPS is composed of branched pentasaccharide K-units containing two D-Glcp residues, and one residue each of D-GalpNAc, D-GlcpNAc and D-Galp. The following structure of the pentasaccharide repeating unit of the CPS was established:

             b-D-Glcp-(1-3)-+             
                            |             
-2)-a-D-Galp-(1-6)-a-D-Glcp-(1-4)-a-D-GalpNAc-(1-3)-b-D-GlcpNAc-(1-

Functions of genes in the K locus of A. baumannii K104 were assigned by a comparison with sequences in the available databases and found to be in agreement with the CPS structure.

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Дом : Наука