STRUCTURE ELUCIDATION AND GENE CLUSTER CHARACTERIZATION OF THE O-ANTIGEN OF YERSINIA KRISTENSENII C-134

O.V. Sizova^a, A.S. Shashkov^a, Ph.V. Toukach^a, Y.A. Knirel^a, R.Z. Shaikhutdinova^b, S.A. Ivanov^a, A.A. Kislichkina^b, S.V. Dentovskaya^b*

^aN. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia
^bState Research Center for Applied Microbiology and Biotechnology, 142279 Obolensk, Russia

KEYWORDS: Yersinia kristensenii; O-specific polysaccharide; O-antigen; Lipopolysaccharide; Glycerol 1-phosphate; Bacterial polysaccharide structure

Carbohydrate Research, 2019, v. 481, pp. 9-15

DOI: 10.1016/j.carres.2019.06.001, PMID: 31220629

Mild acid degradation of the lipopolysaccharide of Yersinia kristensenii C-134 afforded a glycerol teichoic acid-like O-polysaccharide, which was studied by sugar analysis, O-deacetylation and dephosphorylation along with 1D and 2D NMR spectroscopy. The following structure of the O-polysaccharide was established:

      Ac       a-D-Glcp-(1-6)-a-D-Galp(1        a-D-Glcp(1
      |3                               |3                |6
-2)-D-gro-1P-(O-6)-b-D-Glcp-(1-4)-a-L-FucpNAc-(1-3)-b-D-GlcpNAc-(1

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