Abstract: NMR structural studies of Proteus vulgaris O44 O-antigen

STRUCTURE OF THE O-POLYSACCHARIDE OF PROTEUS VULGARIS O44:
A NEW O-ANTIGEN THAT CONTAINS AN AMIDE OF D-GLUCURONIC ACID WITH L-ALANINE

F.V. Toukach^a, A.V. Perepelov^a, B. Bartodziejska,^b, A.S. Shashkov^a, A. Blaszczyk^b, N.P. Arbatsky^a, A. Rozalski^b, Y.A. Knirel^a

^aN. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
^bCenter of Microbiology and Virology, Polish Academy of Sciences, Lodz, Poland

KEYWORDS: Proteus vulgaris, O-Antigen, Lipopolysaccharide, Polysaccharide structures, N-(D-Glucuronoyl)-L-alanine

Carbohydrate Research, 2003, v. 338, pp. 1431-1435

DOI: 10.1016/S0008-6215(03)00096-X

The O-polysaccharide of Proteus vulgaris O44, strain PrK 67/57 was studied by ¹H and ¹³C NMR spectroscopy, including 2D COSY, TOCSY, ROESY, H-detected ¹H,¹³C HMQC, HMQC/TOCSY and HMBC experiments. The polysaccharide was found to contain an amide of D-glucuronic acid with L-alanine, and the following structure of the linear pentasaccharide repeating unit was established:

       l-Ala
	 |6
-4)bDGlcpA6(1-3)bDGalpNAc(1-4)bDGlcp(1-3)aDGalp(1-4)bDGalpNAc(1-

The structural data of the O-polysaccharide and the results of serological studies with P. vulgaris O44 O-antiserum showed that the strain studied is unique among Proteus bacteria, which is in agreement with its classification in a separate Proteus serogroup, O44.

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STRUCTURE OF THE O-POLYSACCHARIDE OF PROTEUS VULGARIS O44: A NEW O-ANTIGEN THAT CONTAINS AN AMIDE OF D-GLUCURONIC ACID WITH L-ALANINE

STRUCTURE OF THE O-POLYSACCHARIDE OF PROTEUS VULGARIS O44:
A NEW O-ANTIGEN THAT CONTAINS AN AMIDE OF D-GLUCURONIC ACID WITH L-ALANINE