STRUCTURE OF THE NEW ACIDIC O-ANTIGEN OF PROTEUS VULGARIS O22 CONTAINING O-ACETYLATED 3-ACETAMIDO-3,6-DIDEOXY-D-GLUCOSE

F.V. Toukach^a, B. Bartodziejska^b, S.N. Senchenkova^a, M. Wykrota^b, A.S. Shashkov^a, A. Rozalski^b, Y.A. Knirel^a

^aN. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
^bCenter of Microbiology and Virology, Polish Academy of Sciences, Lodz, Poland

KEYWORDS: Proteus vulgaris, O-antigen, O-specific polysaccharide, lipopolysaccharide, O-acetylation, 3-acetamido-3,6-dideoxy-D-glucose

Carbohydrate Research, 1999, v. 318(1-4), pp. 146-153

The acidic O-specific polysaccharide of Proteus vulgaris O22 was studied using ¹H and ¹³C NMR spectroscopy, including 2D COSY, TOCSY, NOESY, and H-detected ¹H,¹³C heteronuclear multiple-quantum coherence (HMQC) experiments, and the following structure of the branched pentasaccharide repeating unit was established:

65% 2-OAc, 4-OAc-aDQuip3NAc
                   |3
-3)bDGlcpNAc(1-2)bLRhap(1-4)aLRhap(1-4)bDGlcpA(1-

where Qui3NAc is 3-acetamido-3,6-dideoxyglucose, O-acetylation of QuiNAc at position 4 is stoichiometric and at position 2 nonstoichiometric. Serological relationships of P. vulgaris O22 with some other Proteus strains was substantiated on the level of the O-antigen structures.

Science: Proteus

Publications: Proteus

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