STRUCTURAL AND SEROLOGICAL STUDIES OF THE RELATED O-SPECIFIC POLYSACCARIDES OF PROTEUS VULGARIS O21 AND PROTEUS MIRABILIS O48 HAVING OLIGOSACCHARIDE-PHOSPHATE REPEATING UNITS

B. Bartodziejska^a, F.V. Toukach^b, E.V. Vinogradov^c, S.N. Senchenkova^b, A.S. Shashkov^b, A. Ziolkowski^a, J. Czaja^d, M.B. Perry^c, Y.A. Knirel^b, A. Rozalski^a

^aDepartment of Immunobiology of Bacteria, Institute of Microbiology and Immunology, University of Lodz, Poland
^bN. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
^cInstitute of Biological Sciences, National research Council, Ottawa, Canada
^dL. Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, Wroclaw, Poland

KEYWORDS: lipopolysaccharide, O-specific polysaccharide structure, oligosaccharide phosphate, Proteus, serological cross-reactivity

Eur. J. Biochem, 2000, v. 267, pp.6888-6896

DOI: 10.1046/j.1432-1033.2000.01793.x    (free full text)

The O-specific polysaccharide chains (O-antigens) of the lipopolysaccharides (LPSs) of Proteus mirabilis O48 and Proteus vulgaris O21 were found to have tetrasaccharide and pentasaccharide repeating units, respectively, interlinked by a glycosidic phosphate. Polysaccharides and an oligosaccharide were derived from the LPSs by various degradation procedures and studied by ¹H and ¹³C NMR spectroscopy, including 2D COSY, TOCSY, NOESY, H-detected ¹H,¹³C and ¹H,³¹P HMQC experiments. The following related structures of the repeating units of the O-antigens were established:

-4)aGalpNAc(1-3)bGalpNAc(1-2)aGalp(1-P-6)[AcO-3]aGlcpNAc(1-  (P.v. O48)

-4)[aGlcp(1-6)]aGalpNAc(1-3)bGalpNAc(1-2)aGlcp(1-P-6)aGlcpNAc(1-  (P.v. O21)

The O-specific polysaccharide of P. vulgaris O21 has the same structure as that of Hafnia alvei 744 and PCM 1194 [...], except that the GlcN residue carries the N-acetyl rather than the N-[(R)-3-hydroxybutyryl] group. Serological investigations confirmed the close relatedness of the Proteus and Hafnia O-antigens studied.

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