THE O-POLYSACCHARIDE FROM THE LIPOPOLYSACCHARIDE OF PROVIDENCIA STUARTII O44 CONTAINING L-QUINOVOSE, A 6-DEOXY SUGAR RARELY UCCURRING IN BACTERIAL POLYSACCHARIDES

Nina A. Kocharova^a, Olga G. Ovchinnikova^a*, Filip V. Toukach^a, Agnieszka Torzewska^b, Alexander S. Shashkov^a, Yuriy A. Knirel^a and Antoni Rozalski^b

^aN. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
^bDepartment of Immunobiology of Bacteria, Institute of Microbiology and Immunology, University of Lodz, Poland

KEYWORDS: Providencia stuartii, O-antigen, Lipopolysaccharide, Polysaccharide structure

Carbohydrate Research, 2005, v. 340, pp. 1419-1423

DOI: 10.1016/j.carres.2005.02.020

The O-polysaccharide (O-antigen) of Providencia stuartii O44:H4 (strain 3768/51) was obtained by mild acid degradation of the lipopolysaccharide and studied by sugar and methylation analyses along with ¹H and ¹³C NMR spectroscopy, including 2D ¹H,¹H COSY, TOCSY, ROESY and H-detected ¹H,¹³C HSQC and HMQC-TOCSY experiments. The O-polysaccharide was found to have a branched hexasaccharide repeating unit of the following structure:

    bDGlcpA
       |4
->3)aLFucp(1->3)aDGlcp(1->4)aLQuip(1->3)aDGlcpNAc(1->4)aDGalpNAc(1->

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