STRUCTURE OF THE O-SPECIFIC POLYSACCHARIDE OF PROTEUS PENNERI 71 AND CLASSIFICATION OF CROSS-REACTIVE P. PENNERI STRAINS TO A NEW PROPOSED SEROGROUP O64

K. Zych^a, N.A. Kocharova^b, M. Kowalczyk^a, F.V. Toukach^b, D. Kaminska^a, A.S. Shashkov^b, Y.A. Knirel^b, Z. Sidorczyk^a

^aDepartment of General Microbiology, Institute of Microbiology and Immunology, University of Lodz, Poland
^bN. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia

KEYWORDS: Bacterial polysaccharide, O-Antigen, Lipopolysaccharide, Proteus penneri, NMR spectroscopy, serological cross-reactivity

European J. Biochem., 2000, v. 267, pp. 808-814

DOI: 10.1046/j.1432-1327.2000.01059.x    (free full text)

A neutral O-specific polysaccharide (O-antigen) was isolated from the lipopolysaccharide (LPS) of the bacterium Proteus penneri 71. On the basis of sugar analysis and ¹H ¹³C NMR spectroscopic studies including two-dimensional COSY, ¹H,¹³C heteronuclear COSY and ROESY, the following structure of the trisaccharide repeating unit of the polysaccharide was established:

-3)bDGlcpNAc(1-4)bDGlcpNAc(1-3)aDGalp(1-

The polysaccharide has the same carbohydrate backbone as the O-specific polysacccharide of P. penneri 19 and both are similar to that of P. penneri 62 studied by us previously. A cross-reactivity of anti-P. penneri 71, 19 and 62 O-antisera with 11 P. penneri strains was revealed and substantiated at the level of the O-antigen structures. These strains could be divided into three subgroups within a new proposed Proteus O64 serogroup containing P. penneri strains only.

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