STRUCTURE OF THE O-SPECIFIC POLYSACCHARIDE OF THE BACTERIUM PROTEUS MIRABILIS O30

A.S. Shashkov^a, F.V. Toukach^a, A. Ziolkowski^b, N.A. Paramonov^a, S.N. Senchenkova^a, W. Kaca^b, Y.A. Knirel^a*

^aN. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia
^bCenter of Microbiology and Virology, Polish Academy of Sciences, Lodz, Poland

KEYWORDS: lipopolysaccharide, O-antigen, polysaccharide, structure, NMR spectroscopy, Proteus mirabilis

Biochemistry/Moscow, 1996, v. 61(5), pp. 575-579

Based on acid hydrolysis, methylation, and one- and two-dimensional ¹H- and ¹³C-NMR spectroscopy, including homonuclear and ¹H,¹³C heteronuclear correlation spectroscopy (COSY) and rotating-frame nuclear Overhauser effect spectroscopy (ROESY), it was found that the O-specific polysaccharide chain of the lipopolysaccharide of the bacterium Proteus mirabilis O30 is a hexosaminoglucuronan built up of tetrasaccharide repeating units having the following structure:

-4)bDGlcpA(1-6)aDGalpNAc(1-6)bDGlcpNAc(1-3)[70%AcO-4)]bDGlcpNAc(1-

The degree of O-acetylation of 2-acetamido-2-deoxyglucose is about 70%.

Science: Proteus

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