STRUCTURAL CHARACTERISTICS OF WATER-SOLUBLE POLYSACCHARIDES FROM HERACLEUM SOSNOWSKYI MANDEN

E.G. Shakhmatov^a, Ph.V. Toukach^b, S.P. Kuznetsov^a, E.N. Makarova^a

^aInstitute of Chemistry, Komi Science Centre, The Urals Branch of the Russian Academy of Sciences, Syktyvkar, Russia
^bN. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, Moscow, Russia

KEYWORDS: Heracleum sosnowskyi; Arabinogalactan protein; Pectin; Structure; NMR; Glucomannan

Carbohydrate Polymers, 2014, v. 102, pp. 521-528

DOI: 10.1016/j.carbpol.2013.12.001

Fractions containing arabinogalactan proteins (AGP) and pectic polysaccharides were isolated from above-ground parts of Heracleum sosnowskyi. Major units of their structure were elucidated using ion-exchange chromatography, gel chromatography, and NMR spectroscopy. The carbohydrate backbone of the polysaccharides consisted of 1,3-β-D-galactopyranosyl residues, whereas side chains of the branched region consisted of the residues of 1,6-β-galactopyranose, 1,5-α-L-arabinofuranose, 1,4-β-D-glucuronic acid, and 1,6-β-D-glucopyranose. The branching points were identified as 1,3,6-β-D-galactopyranose residues. Side chains were terminated with β-D-galactopyranose, α-L-arabinofuranose and α-L-rhamnopyranose. A significant part of the side-chain β-1,6-galactan was substituted at C6 by 4-ÎÌå-β-D-glucuronic acid. A minor part of glucuronic acid was included in the α-Rhap-(1->4)-β-GlcA-(- fragment. All the studied fractions contained 1,4-β-D-galacturonic acid as well.

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