STRUCTURE AND GENE CLUSTER OF THE CAPSULAR POLYSACCHARIDE PRODUCED BY ACINETOBACTER BAUMANNII K111

A.V. Filatov^a, A.M. Shpirt^a, A.V. Perepelov^a, A.S. Dmitrenok^a, M.M. Shneider^b, P.V. Toukach^a, Y.A. Knirel^a

^aN.D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russia
^bM.M. Shemyakin & Y.A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia

KEYWORDS: Acinetobacter baumannii; Ñapsular polysaccharide; K locus; Structure; Gene cluster

Carbohydrate Research, 2026, v. 559, ID 109760

DOI: 10.1016/j.carres.2025.109760, PMID: 41297482

Structure of the capsular polysaccharide (CPS) from A. baumannii MAR21-2688 strain (classified as K111 type) was established using ¹H and ¹³C NMR spectroscopy, including two-dimensional homonuclear 1Í,1Í COSY, TOCSY, ROESY and heteronuclear 1Í,13C HSQC and HMBC experiments and chemical methods including component analyses and Smith degradation. The following structure of the branched heptasaccharide repeating K-unit was established:

b-D-ManpNAc-(1-2)-a-L-Rhap-(1
                            |
                            2
                   -3)-a-L-Rhap-(1-2)-a-L-Rhap-(1-2)-a-L-Rhap-(1-3)-b-D-GlcpNAc-(1-

Functions of genes in the K locus of A. baumannii K111 were assigned by a comparison with sequences in the available databases and found to be in agreement with the CPS structure.

Publications: Rest

Home : Science